Extracellular Hco3− Dependence of Electrogenic Na/Hco3 Cotransporters Cloned from Salamander and Rat Kidney
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Extracellular Hco3− Dependence of Electrogenic Na/Hco3 Cotransporters Cloned from Salamander and Rat Kidney
We studied the extracellular [HCOabstract (3) (-)] dependence of two renal clones of the electrogenic Na/HCO(3) cotransporter (NBC) heterologously expressed in Xenopus oocytes. We used microelectrodes to measure the change in membrane potential (DeltaV(m)) elicited by the NBC cloned from the kidney of the salamander Ambystoma tigrinum (akNBC) and by the NBC cloned from the kidney of rat (rkNBC)...
متن کاملCotransporters Cloned from Salamander and Rat Kidney
We studied the extracellular [HCO ] dependence of two renal clones of the electrogenic Na/HCO 3 cotransporter (NBC) heterologously expressed in Xenopus oocytes. We used microelectrodes to measure the change in membrane potential ( D V m ) elicited by the NBC cloned from the kidney of the salamander Ambystoma tigrinum (akNBC) and by the NBC cloned from the kidney of rat (rkNBC). We used a two-el...
متن کاملCation and voltage dependence of rat kidney electrogenic Na-HCO3
Sciortino, Christopher M., and Michael F. Romero. Cation and voltage dependence of rat kidney electrogenic Na-HCO3 2 cotransporter, rkNBC, expressed in oocytes. Am. J. Physiol. 277 (Renal Physiol. 46): F611–F623, 1999.— Recently, we reported the cloning and expression of the rat renal electrogenic Na-HCO3 2 cotransporter (rkNBC) in Xenopus oocytes [M. F. Romero, P. Fong, U. V. Berger, M. A. Hed...
متن کاملCloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
We have recently cloned the renal electrogenic Na(+)-bicarbonate contransporter of the salamander Ambystoma tigrinum (aNBC) (M. F. Romero, M. A. Hediger, E. L. Boulpaep, and W. F. Boron. FASEB J. 10: 89, 1996; and Nature 387: 409-413, 1997). Here we report the cloning of a mammalian homolog of aNBC, named rNBC for rat Na(+)-bicarbonate cotransporter. NBC constitutes the major route for HCO3- re...
متن کاملApical electrogenic NaHCO3 cotransport. A mechanism for HCO3 absorption across the retinal pigment epithelium
Intracellular microelectrode techniques and intracellular pH (pHi) measurements using the fluorescent dye 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) were employed to characterize an electrogenic bicarbonate transport mechanism at the apical membrane of the frog retinal pigment epithelium (RPE). Reductions in apical concentrations of both [HCO3]o (at constant Pco2 or pHo) or [Na]o c...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2000
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.115.5.533